Fig. 1

Validation of the PoE platform and assay. a Comparison of the efficiency of extraction of Ebola virus stocks H.sapiens-tc/COD/1995/Kikwit-15476 (EBOV/Kik) between the M1 Sample Prep or the Qiagen Viral RNA kit. The amount of RNA extracted by the two methods was not statistically different (Mann-Whitney test p > 0.05). Displayed are the three individual replicates for each extraction including mean and standard error of the mean. b Comparison of the Biomeme Franklin Three9 Real-Time PCR Thermocycler (BM) and the Smart Cycler using a ten-fold dilution series of EBOV/Kik, extracted using the Biomeme M1 Sample Prep, and measured using Roche LightCycler 480 RNA Master Hydrolysis Probes. c Comparison of the Biomeme lyophilized reagent system with the Roche LightCycler 480 RNA Master Hydrolysis Probes reagents and the Altona RealStar Ebola virus RT-PCR kit. A ten-fold dilution series was analyzed using the three reagent systems on the BM. The results of each individual replicate are depicted in the graphs. Genome copies/μL were calculated using a linear regression model derived from a tenfold dilution series analyzed with Droplet Digital PCR